I understand using tetracycline resistance to select for transformed cells, but won't cells that have this new genome still be transcribing their original genome? How is the original genome lost after a few rounds of cell division, and how do cells divide with two separate genomes?
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I understand using tetracycline resistance to select for transformed cells, but won't cells that have this new genome still be transcribing their original genome? How is the original genome lost after a few rounds of cell division, and how do cells divide with two separate genomes?
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